STK35 Antikörper (C-Term)

Produktnummer ABIN185178

Dieses Anti-STK35-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von STK35 in WB, ELISA, IHC, IF und FACS. Geeignet für Human, Maus und Schwein. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für STK35 Antikörper (C-Term) (ABIN185178)

Target: STK35 (Serine/threonine Kinase 35 (STK35))

Reaktivität: Human, Maus, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser STK35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-STK35 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Clik1 / STK35

Sequenz: ELETRMDQVT CAA

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-STK35 Antikörper (ABIN185178)

Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

Details zu STK35

Target: STK35 (Serine/threonine Kinase 35 (STK35))

Andere Bezeichnung: STK35

Hintergrund: STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form

Gen-ID: 140901, 67333

NCBI Accession: NP_543026