Pleckstrin Antikörper (C-Term)

Produktnummer ABIN185127

Dieses Anti-Pleckstrin-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Pleckstrin in WB, IHC, ELISA, FACS und IF. Geeignet für Human.

Kurzübersicht für Pleckstrin Antikörper (C-Term) (ABIN185127)

Target: Pleckstrin (PLEK)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Pleckstrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Pleckstrin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Pleckstrin

Sequenz: EWIKAIQMAS RTGK

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EWIKAIQMASRTGK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40-45 kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1 kDa according to Human NP_002655.2). Recommended concentration: 0.0

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Pleckstrin

Target: Pleckstrin (PLEK)

Andere Bezeichnung: PLEK

Hintergrund: PLEK, pleckstrin, P47, p47, FLJ27168

Gen-ID: 5341

NCBI Accession: NP_002655

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process