BOLL Antikörper (C-Term)

Produktnummer ABIN185072

Der Ziege Polyklonal Anti-BOLL-Antikörper wurde für ELISA und IHC validiert. Er ist geeignet, BOLL in Proben von Human zu detektieren.

Kurzübersicht für BOLL Antikörper (C-Term) (ABIN185072)

Target: BOLL (Bol, Boule-Like (BOLL))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BOLL Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-BOLL Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BOULE / BOLL

Sequenz: QPEPIKTVWS IHY

Spezifität: This antibody is expected to recognize both reported isoforms (NP_932074.1, NP_149019.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1, NP_149019.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 22 kDa band in Human Testis lysates after 1.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu BOLL

Target: BOLL (Bol, Boule-Like (BOLL))

Andere Bezeichnung: BOLL

Hintergrund: BOLL, bol, boule-like (Drosophila), BOULE, boule

Gen-ID: 66037

NCBI Accession: NP_932074, NP_149019