PPP1R8 Antikörper (C-Term)

Produktnummer ABIN185050

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PPP1R8 in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für PPP1R8 Antikörper (C-Term) (ABIN185050)

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPP1R8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-PPP1R8 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NIPP1 / PPP1R8

Sequenz: EAWPGKKPTP SLLI

Spezifität: This antibody is expected to recognise all three human isoforms of this protein.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8 μg/mL.

Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5 kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 h

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PPP1R8

Target: PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))

Andere Bezeichnung: PPP1R8

Hintergrund: PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory

Gen-ID: 5511, 100336

NCBI Accession: NP_002704, NP_054829, NP_612568