TRIM2 Antikörper (C-Term)

Produktnummer ABIN184974

Dieses Anti-TRIM2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRIM2 in WB, ELISA, FACS und IHC. Geeignet für Human und Ratte.

Kurzübersicht für TRIM2 Antikörper (C-Term) (ABIN184974)

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-TRIM2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: TRIM2

Sequenz: DSGNHCFKVY RYLQ

Spezifität: Cross-reactivity with TRIM3 has been demonstrated in Mouse.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2, NP_001123539.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12 μg/mL.

Western Blot: Approx 75-85 kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation wi

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRIM2

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Andere Bezeichnung: TRIM2

Hintergrund: TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2

Gen-ID: 23321, 80890

NCBI Accession: NP_056086, NP_001123539, NP_001337983, NP_001337984, NP_001337986