TRIM55 Antikörper (C-Term)

Produktnummer ABIN184953

Dieses Anti-TRIM55-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRIM55 in WB, ELISA, IHC, FACS und IF. Geeignet für Human.

Kurzübersicht für TRIM55 Antikörper (C-Term) (ABIN184953)

Target: TRIM55 (Tripartite Motif Containing 55 (TRIM55))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM55 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-TRIM55 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: MURF2 / TRIM55

Sequenz: HIFSFSWLNS LNE

Spezifität: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).

Kreuzreaktivität: Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRIM55

Target: TRIM55 (Tripartite Motif Containing 55 (TRIM55))

Andere Bezeichnung: TRIM55

Hintergrund: TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2

Gen-ID: 84675, 381485, 365751

NCBI Accession: NP_908973, NP_908974, NP_908975