PSMF1 Antikörper (C-Term)

Produktnummer ABIN184933

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PSMF1 in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PSMF1 Antikörper (C-Term) (ABIN184933)

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PSMF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-PSMF1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PI31 / PSMF1 (Isoform 1)

Sequenz: DHLPPPGYDD MYL

Spezifität: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DHLPPPGYDDMYL

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293, approx 30-32 kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35 kDa in lysates of cell line LNCaP (calculated MW of 29.8 kDa according to Human NP_006805.2 an

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry:A customer reported weak staining of A431 cells at

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PSMF1

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Andere Bezeichnung: PSMF1

Hintergrund: PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1

Gen-ID: 9491, 228769

NCBI Accession: NP_006805

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA