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CHFR Antikörper (C-Term)

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CHFR in ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN184918

Kurzübersicht für CHFR Antikörper (C-Term) (ABIN184918)

Target

Alle CHFR Antikörper anzeigen
CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reaktivität

  • 42
  • 15
  • 14
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 39
  • 3
Ziege

Klonalität

  • 39
  • 3
Polyklonal

Konjugat

  • 20
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser CHFR Antikörper ist unkonjugiert

Applikation

  • 23
  • 22
  • 8
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC)

Güteklasse

Verified
  • Bindungsspezifität

    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Verwendungszweck

    CHFR

    Sequenz

    KFNHICEQTR FKN

    Spezifität

    This antibody is expected to recognise all reported isoforms.

    Kreuzreaktivität

    Rind (Kuh), Human, Maus, Schwein, Ratte

    Aufreinigung

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1, NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5 μg/mL.

    Western Blot: Preliminary experiments gave an approx 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Minimize freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

    Andere Bezeichnung

    CHFR

    Hintergrund

    CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196

    Gen-ID

    55743, 231600, 288734

    NCBI Accession

    NP_001154816, NP_001154817, NP_001154818, NP_060693, NP_001154819
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