ARIH2 Antikörper (C-Term)

Produktnummer ABIN184916

Der Ziege Polyklonal Anti-ARIH2-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, ARIH2 in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ARIH2 Antikörper (C-Term) (ABIN184916)

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARIH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ARIH2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ARIH2 / TRIAD1

Sequenz: EQRRRTLLKD FHDT

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ARIH2 Antikörper (ABIN184916)

Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

Details zu ARIH2

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Andere Bezeichnung: ARIH2

Hintergrund: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2

Gen-ID: 10425, 23807

NCBI Accession: NP_006312