PUF60 Antikörper (C-Term)

Produktnummer ABIN184855

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PUF60 in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PUF60 Antikörper (C-Term) (ABIN184855)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PUF60 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-PUF60 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SIAHBP1 / FIR

Sequenz: YDQERFDNSD LSA

Spezifität: This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1, NP_055096.2, NP_001129505.1)

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YDQERFDNSDLSA, from the C Terminus of the protein sequence according to NP_510965.1, NP_055096.2, NP_001129505.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate and Skin. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human lysates of HEK293 (calculated MW of 59.9 kDa according to NP_510965.1 and 58.2 according to NP_055096.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Andere Bezeichnung: PUF60

Hintergrund: PUF60, SIAHBP1, FIR, fuse-binding protein-interacting repressor, RoBPI, siah binding protein 1, poly-U binding splicing factor PUF60, Ro ribonucleoprotein-binding protein 1, pyrimidine tract binding splicing factor, poly-U binding splicing factor 60KDa, F

Gen-ID: 22827, 67959

NCBI Accession: NP_510965, NP_055096, NP_001129505