ARL6IP5 Antikörper (C-Term)

Produktnummer ABIN184829

Der Ziege Polyklonal Anti-ARL6IP5-Antikörper wurde für WB, ELISA, IHC, IF und FACS validiert. Er ist geeignet, ARL6IP5 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ARL6IP5 Antikörper (C-Term) (ABIN184829)

Target: ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARL6IP5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ARL6IP5 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: GTRAP3-18 / JWA

Sequenz: NRLTDYISKV KE

Spezifität: C2 passed G1 as 20 kDa in HBR, C2P2 fail in _enh, try HBAM, HBCR, HBFC BP08/10/2021, 22+48 kDa in HBAM-B104, faint 48 kDa in HBAM-B201, multiple bannd in > 37 kDa in both HBCR, multiple bands inclu 22 kDa in both HBFC, opt in HBAM-B201 at 2 μg/mL , rpt HBAM-B10

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NRLTDYISKVKE, from the C Terminus of the protein sequence according to NP_006398.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 22 kDa band observed in Human Cerebellumn lysates and in lysates of cell line K562 (calculated MW of 21.6 kDa according to NP_006398.1). An additional band of 75-100 kDa was also observed in some lysates.and was successfully blocked by

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the ER/ plasma membranes and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ARL6IP5 Antikörper (ABIN184829)

Wang, Gong, Chen, Liu, Li, Li, Zhou: "JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks." in: Nucleic acids research, Vol. 37, Issue 6, pp. 1936-50, (2009) (PubMed).

Details zu ARL6IP5

Target: ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

Andere Bezeichnung: ARL6IP5

Hintergrund: ARL6IP5, GTRAP3-18, JWA, ADP-ribosylation-like factor 6 interacting protein 5, DERP11, HSPC127, PRAF3, addicsin, hp22, jmx, PRA1 domain family 3, cytoskeleton related vitamin A responsive protein, dermal papilla derived protein 11, glutamate transporter E

Gen-ID: 10550

NCBI Accession: NP_006398

Pathways: Dicarboxylic Acid Transport