ARL2 Antikörper (C-Term)

Produktnummer ABIN184799

Der Ziege Polyklonal Anti-ARL2-Antikörper wurde für WB, ELISA, FACS und IF validiert. Er ist geeignet, ARL2 in Proben von Human zu detektieren.

Kurzübersicht für ARL2 Antikörper (C-Term) (ABIN184799)

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ARL2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ARL2

Sequenz: LDDISSRIFT AD

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LDDISSRIFTAD, from the C Terminus of the protein sequence according to NP_001658.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 18 kDa band observed in lysates of cell line A431 and approx. 20 kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9 kDa according to NP_001658.2). Recommended concentration: 0.5-1 μg/mL.Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ARL2

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Andere Bezeichnung: ARL2

Hintergrund: ARL2, ADP-ribosylation factor-like 2, ARFL2, ADP=ribosylation factor-like 2

Gen-ID: 402

NCBI Accession: NP_001658

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location