AIRE Antikörper (C-Term)

Produktnummer ABIN184780

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch AIRE in WB, ELISA, IF, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für AIRE Antikörper (C-Term) (ABIN184780)

Target: AIRE (Autoimmune Regulator (AIRE))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AIRE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-AIRE Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: AIRE

Sequenz: QSMARPAAPF PS

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. R

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Andere Bezeichnung: AIRE

Hintergrund: AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop

Gen-ID: 326

NCBI Accession: NP_000374

Pathways: Chromatin Binding