AIRE Antikörper (C-Term)
Kurzübersicht für AIRE Antikörper (C-Term) (ABIN184780)
Target
Alle AIRE Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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    - 
                                            Bindungsspezifität
- C-Term
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                                            Verwendungszweck
- AIRE
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                                            Sequenz
- QSMARPAAPF PS
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                                            Kreuzreaktivität
- Human
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                                            Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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                                            Immunogen
- Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.
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                                            Isotyp
- IgG
 
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    - 
                                            Applikationshinweise
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                        Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL. Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t Peptide ELISA: antibody detection limit dilution 1:128000. 
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                                            Kommentare
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                        Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. R 
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                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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    - 
                                            Format
- Liquid
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                                            Konzentration
- 0.5 mg/mL
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                                            Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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                                            Konservierungsmittel
- Sodium azide
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                                            Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            Handhabung
- Minimize freezing and thawing.
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                                            Lagerung
- -20 °C
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                                            Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
 
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    - AIRE (Autoimmune Regulator (AIRE))
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                                            Andere Bezeichnung
- AIRE
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                                            Hintergrund
- AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop
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                                            Gen-ID
- 326
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                                            NCBI Accession
- NP_000374
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                                            Pathways
- Chromatin Binding
 Target
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