JUP Antikörper (C-Term)

Produktnummer ABIN184775

Der Ziege Polyklonal Anti-JUP-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, JUP in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für JUP Antikörper (C-Term) (ABIN184775)

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser JUP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-JUP Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Plakoglobin / Gamma-catenin

Sequenz: GLRPPYPTAD HMLA

Spezifität: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Kreuzreaktivität: Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: JUP

Hintergrund: JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa

Gen-ID: 3728, 16480

NCBI Accession: NP_002221, NP_068831

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location