KIF4A Antikörper (C-Term)

Produktnummer ABIN184768

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch KIF4A in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für KIF4A Antikörper (C-Term) (ABIN184768)

Target: KIF4A (Kinesin Family Member 4A (KIF4A))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIF4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-KIF4A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: KIF4A

Sequenz: SFFSGCSPIE EEAH

Kreuzreaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SFFSGCSPIEEEAH, from the C Terminus of the protein sequence according to NP_036442.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 150 kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5 kDa according to Mouse NP_032472.2). Recommended concentration:1-2 μg/mL. Primary incubation 1 hour at room temperature. Negative Control:

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu KIF4A

Target: KIF4A (Kinesin Family Member 4A (KIF4A))

Andere Bezeichnung: KIF4A

Hintergrund: KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4

NCBI Accession: NP_036442