ELMO2 Antikörper (C-Term)

Produktnummer ABIN184684

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ELMO2 in WB, ELISA, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für ELMO2 Antikörper (C-Term) (ABIN184684)

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ELMO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ELMO2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ELMO2

Sequenz: PKEPSSYDFV YHYG

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-PKEPSSYDFVYHYG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6 kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7 kDa according

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ELMO2

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Andere Bezeichnung: ELMO2

Hintergrund: ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2

Gen-ID: 63916, 140579

NCBI Accession: NP_877496, NP_573403