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FOXP3 Antikörper (C-Term)

Name: FOXP3 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN184677
Price: 575.06 EUR
Availability: InStock

Dieses Anti-FOXP3-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von FOXP3 in WB, FACS, ELISA, IHC, IF und ICC. Geeignet für Human.

ABIN184677 (1µg/ml) staining of Human Muscle (A), (2ug/ml) MOLT4 (B) and (1µg/ml) negative control Pancreas (C) lysate (35µg protein in RIPA buffer). (FOXP3 Antikörper (C-Term))

ABIN184677 (1µg/ml) staining of paraffin embedded Human Tonsil. (FOXP3 Antikörper (C-Term))

IHC

ABIN184677 staining of CD25-sorted (Treg) Human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B). (FOXP3 Antikörper (C-Term))

ICC

ABIN184677 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (FOXP3 Antikörper (C-Term))

ABIN184677 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0. (FOXP3 Antikörper (C-Term))

FACS

5 Abbildungen

Produktnummer ABIN184677

Datenblatt (PDF)

Kurzübersicht für FOXP3 Antikörper (C-Term) (ABIN184677)

Target

Alle FOXP3 Antikörper anzeigen

FOXP3 (Forkhead Box P3 (FOXP3))

Reaktivität

Human

Wirt

Ziege

Klonalität

Polyklonal

Konjugat

Dieser FOXP3 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Güteklasse

Verified

Discover our top product FOXP3 Primärantikörper

Bindungsspezifität
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C-Term

Verwendungszweck

FOXP3 / SCURFIN

Sequenz

SQRPSRCSNP TPGP

Spezifität

This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

Kreuzreaktivität

Human

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen

Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

Isotyp

IgG
anti-Forkhead Box P3 (FOXP3) (AA 190-412) antibody
FOXP3 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Forkhead Box P3 (FOXP3) (C-Term) antibody
Verified FOXP3 Reaktivität: Maus FACS, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated

anti-Forkhead Box P3 (FOXP3) (AA 297-431) antibody
FOXP3 Reaktivität: Human FACS, ELISA, IHC Wirt: Maus Monoclonal 4F12A2 unconjugated

anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibody
FOXP3 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Forkhead Box P3 (FOXP3) (N-Term) antibody
FOXP3 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Pferd, Hund, Schaf, Meerschweinchen WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibody
FOXP3 Reaktivität: Human WB, IHC, IP, ICC Wirt: Maus Monoclonal D1 unconjugated

anti-Forkhead Box P3 (FOXP3) (AA 283-311), (C-Term) antibody
FOXP3 Reaktivität: Human, Maus WB, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB17111 unconjugated

anti-Forkhead Box P3 (FOXP3) antibody (APC)
FOXP3 Reaktivität: Human, Maus ICFC Wirt: Maus Monoclonal 3G3 APC

anti-Forkhead Box P3 (FOXP3) (AA 1-431) antibody
FOXP3 Reaktivität: Human WB, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Forkhead Box P3 (FOXP3) (AA 101-431) antibody
FOXP3 Reaktivität: Human, Maus, Ratte IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat
Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung

FOXP3

Hintergrund

FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin

Gen-ID

50943

NCBI Accession

NP_054728, NP_001107849

Pathways

Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation