ALDH1A1 Antikörper (C-Term)
Kurzübersicht für ALDH1A1 Antikörper (C-Term) (ABIN184581)
Target
Alle ALDH1A1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- C-Term
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Verwendungszweck
- ALDH1A1 (C Terminus)
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Sequenz
- EVKTVTVKIS QKNS
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Spezifität
- This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.
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Kreuzreaktivität
- Human, Maus, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- C-EVKTVTVKISQKNS
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Isotyp
- IgG
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Applikationshinweise
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Western Blot: Approx 55 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:1000.
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Kommentare
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Human, PMID: 32157826.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients." in: Journal of cachexia, sarcopenia and muscle, Vol. 11, Issue 4, pp. 1047-1069, (2020) (PubMed).
: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).
: "
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Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients." in: Journal of cachexia, sarcopenia and muscle, Vol. 11, Issue 4, pp. 1047-1069, (2020) (PubMed).
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- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
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Andere Bezeichnung
- ALDH1A1
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Hintergrund
- ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic
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Gen-ID
- 216, 11668, 24188
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NCBI Accession
- NP_000680
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Pathways
- Dopaminergic Neurogenesis
Target
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