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ALDH1A1 Antikörper (C-Term)

Dieses Anti-ALDH1A1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ALDH1A1 in WB, ELISA und IF. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.
Produktnummer ABIN184581

Kurzübersicht für ALDH1A1 Antikörper (C-Term) (ABIN184581)

Target

Alle ALDH1A1 Antikörper anzeigen
ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reaktivität

  • 115
  • 55
  • 31
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 93
  • 33
  • 2
Ziege

Klonalität

  • 84
  • 44
Polyklonal

Konjugat

  • 69
  • 8
  • 7
  • 5
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ALDH1A1 Antikörper ist unkonjugiert

Applikation

  • 101
  • 63
  • 45
  • 44
  • 36
  • 26
  • 19
  • 14
  • 14
  • 14
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse

Verified
  • Bindungsspezifität

    • 16
    • 13
    • 12
    • 9
    • 8
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Verwendungszweck

    ALDH1A1 (C Terminus)

    Sequenz

    EVKTVTVKIS QKNS

    Spezifität

    This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.

    Kreuzreaktivität

    Human, Maus, Ratte

    Aufreinigung

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    C-EVKTVTVKISQKNS

    Isotyp

    IgG
  • Applikationshinweise

    Western Blot: Approx 55 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:1000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml. This antibody has been successfully used in IF on Human, PMID: 32157826.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Minimize freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Etienne, Joanne, Catelain, Riveron, Bayer, Lafable, Punzon, Blot, Agbulut, Vilquin: "Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients." in: Journal of cachexia, sarcopenia and muscle, Vol. 11, Issue 4, pp. 1047-1069, (2020) (PubMed).

    Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." in: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

  • Target

    ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

    Andere Bezeichnung

    ALDH1A1

    Hintergrund

    ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic

    Gen-ID

    216, 11668, 24188

    NCBI Accession

    NP_000680

    Pathways

    Dopaminergic Neurogenesis
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