BCL7A Antikörper (C-Term)

Produktnummer ABIN184562

Der Ziege Polyklonal Anti-BCL7A-Antikörper wurde für WB, ELISA, IF und FACS validiert. Er ist geeignet, BCL7A in Proben von Human zu detektieren.

Kurzübersicht für BCL7A Antikörper (C-Term) (ABIN184562)

Target: BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BCL7A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-BCL7A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BCL7A

Sequenz: MKLEASQQNS EEM

Spezifität: This antibody is expected to recognize both reported isoforms (NP_066273.1, NP_001019979.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-MKLEASQQNSEEM, from the C Terminus of the protein sequence according to NP_066273.1, NP_001019979.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 26 kDa band observed in Human Lymph Node lysates (calculated MW of 25.0 kDa according to NP_066273.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu BCL7A

Target: BCL7A (B-Cell CLL/lymphoma 7A (BCL7A))

Andere Bezeichnung: BCL7A

Hintergrund: BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7

Gen-ID: 605, 77045

NCBI Accession: NP_066273, NP_001019979