NOTCH2 Antikörper

Produktnummer ABIN6796405

Der Hamster Monoklonal anti-NOTCH2 Antikörper wird verwendet zum Nachweis von NOTCH2 in Proben von Maus und Ratte. Er wurde validiert für FACS.

Kurzübersicht für NOTCH2 Antikörper (ABIN6796405)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Maus, Ratte

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser NOTCH2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: HMN2-35

Produktdetails anti-NOTCH2 Antikörper

Spezifität: Recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Signalling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukaemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. Clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

Aufreinigung: Protein G purified

Immunogen: Mouse Notch 2-Fc fusion protein.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: NOTCH2 / NOTCH-2

Hintergrund: Name/Gene ID: NOTCH2

Synonyms: NOTCH2, AGS2, HN2, Notch (Drosophila) homolog 2, Notch homolog 2 (Drosophila), Notch 2, Notch homolog 2, HJCYS

Gen-ID: 4853

UniProt: Q04721

Pathways: Notch Signalweg, Stem Cell Maintenance