MHC, Class I Antikörper

Produktnummer ABIN181881

Der Maus Monoklonal anti-MHC, Class I Antikörper (Klon 2G5) (ABIN181881) detektiert spezifisch MHC, Class I in FACS und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Rind (Kuh), Meerschweinchen, Schwein, Schaf und Hamster.

Kurzübersicht für MHC, Class I Antikörper (ABIN181881)

Target: MHC, Class I

Reaktivität: Human, Maus, Rind (Kuh), Meerschweinchen, Schwein, Schaf, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC, Class I Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: 2G5

Produktdetails anti-MHC, Class I Antikörper

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Purified H-2Keb and H-2Deb MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Flow Cytometry: 1/10 - 1/25, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MHC, Class I

Target: MHC, Class I

Andere Bezeichnung: MHC Class I

Hintergrund: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I