CD68 Antikörper

Produktnummer ABIN181836

Dieses Anti-CD68-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD68 in FACS, WB, IF, IHC (p), IP und IHC (fro). Geeignet für Maus. Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für CD68 Antikörper (ABIN181836)

Target: CD68 (CD68 Molecule (CD68))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD68 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: FA-11

Produktdetails anti-CD68 Antikörper

Spezifität: This monoclonal FA-11 antibody does detect surface Macrosialin at low levels in resident Mouse peritoneal Macrophages which can be enhanced with thioglycollate stimulation.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Purified Concanavalin A acceptor glycoprotein from P815 cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western Blot. Immunoprecipiation. Immunohistochemistry on Frozen Sections. Flow Cytometry: 1/50-1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-CD68 Antikörper (ABIN181836)

Modenbach, Möller, Asgarbeik, Geist, Rimkus, Dörr, Wolfgramm, Steil, Susemihl, Graf, Schmöker, Böttcher, Hammer, Glaubitz, Lammers, Delcea, Völker, Aghdassi, Lerch, Weiss, Bornscheuer, Sendler: "Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis." in: Nature communications, Vol. 16, Issue 1, pp. 1702, (2025) (PubMed).

Wilden, Glaubitz, Otto, Biedenweg, Nauck, Mack, Ribback, Bröker, von Rheinbaben, Lerch, Aghdassi, Weiss, Sendler: "Mobilization of CD11b+/Ly6chi monocytes causes multi organ dysfunction syndrome in acute pancreatitis." in: Frontiers in immunology, Vol. 13, pp. 991295, (2022) (PubMed).

Sendler, van den Brandt, Glaubitz, Wilden, Golchert, Weiss, Homuth, De Freitas Chama, Mishra, Mahajan, Bossaller, Völker, Bröker, Mayerle, Lerch: "NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis." in: Gastroenterology, Vol. 158, Issue 1, pp. 253-269.e14, (2020) (PubMed).

Asdonk, Steinmetz, Krogmann, Ströcker, Lahrmann, Motz, Paul-Krahe, Flender, Schmitz, Barchet, Hartmann, Nickenig, Zimmer: "MDA-5 activation by cytoplasmic double-stranded RNA impairs endothelial function and aggravates atherosclerosis." in: Journal of cellular and molecular medicine, Vol. 20, Issue 9, pp. 1696-705, (2017) (PubMed).

Jehle, Hoyer, Schöne, Pfeifer, Schild, Jenniches, Bindila, Lutz, Lütjohann, Zimmer, Nickenig: "Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146267, (2016) (PubMed).

Mareninova, Sendler, Malla, Yakubov, French, Tokhtaeva, Vagin, Oorschot, Lüllmann-Rauch, Blanz, Dawson, Klumperman, Lerch, Mayerle, Gukovsky, Gukovskaya: "Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis." in: Cellular and molecular gastroenterology and hepatology, Vol. 1, Issue 6, pp. 678-694, (2015) (PubMed).

Sendler, Beyer, Mahajan, Kauschke, Maertin, Schurmann, Homuth, Völker, Völzke, Halangk, Wartmann, Weiss, Hegyi, Lerch, Mayerle: "Complement Component 5 Mediates Development of Fibrosis, via Activation of Stellate Cells, in 2 Mouse Models of Chronic Pancreatitis." in: Gastroenterology, Vol. 149, Issue 3, pp. 765-76.e10, (2015) (PubMed).

Zimmermann, Homann, Bangert, Müller, Hristov, Goeser, Wiehe, Zittrich, Rottbauer, Torzewski, Pfitzer, Katus, Kaya: "Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis." in: Journal of the American Heart Association, Vol. 1, Issue 6, pp. e003293, (2013) (PubMed).

Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).

Details zu CD68

Target: CD68 (CD68 Molecule (CD68))

Andere Bezeichnung: CD68

Hintergrund: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin

Gen-ID: 12514

NCBI Accession: NP_033983

UniProt: P31996