MIA2 Antikörper (Biotin)

Produktnummer ABIN181709

Der Kaninchen Polyklonal anti-MIA2 Antikörper wird verwendet zum Nachweis von MIA2 in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für MIA2 Antikörper (Biotin) (ABIN181709)

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIA2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-MIA2 Antikörper (Biotin)

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human MIA-2

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanMIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu MIA2

Target: MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Andere Bezeichnung: MIA2

Hintergrund: MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.Synonyms: Melanoma inhibitory activity protein 2

Gen-ID: 9606

UniProt: Q96PC5