beta Defensin 1 Antikörper

Produktnummer ABIN181704

Der Kaninchen Polyklonal Anti-beta Defensin 1-Antikörper wurde für WB und EIA validiert. Er ist geeignet, beta Defensin 1 in Proben von Human zu detektieren.

Kurzübersicht für beta Defensin 1 Antikörper (ABIN181704)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Defensin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-beta Defensin 1 Antikörper

Spezifität: This antibody detects Defensin beta 1.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BD-1

Anwendungsinformationen

Applikationshinweise: ELISA: Indirect: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. This In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-1. Sandwich: To detect hBD-1 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanBD-1 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBD-1. Western blot: To detect hBD-1 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBD-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Andere Bezeichnung: Defensin beta 1

Hintergrund: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

Gen-ID: 1672

NCBI Accession: NP_005209

UniProt: P60022