beta Defensin 1 Antikörper (Biotin)

Produktnummer ABIN181701

Dieses Anti-beta Defensin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von beta Defensin 1 in WB und EIA. Geeignet für Human.

Kurzübersicht für beta Defensin 1 Antikörper (Biotin) (ABIN181701)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Defensin 1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-beta Defensin 1 Antikörper (Biotin)

Spezifität: This antibody detects Human BD-1. Other species not tested.

Aufreinigung: Affinity Chromatography

Immunogen: E. Coli derived recombinant Human beta Defensin-1

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1. Sandwich: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1(AP01151PU) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human BD-1. Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Andere Bezeichnung: Defensin beta 1

Hintergrund: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1

Gen-ID: 1672

NCBI Accession: NP_005209

UniProt: P60022