ARTN Antikörper

Produktnummer ABIN181680

Der Kaninchen Polyklonal Anti-ARTN-Antikörper wurde für WB und EIA validiert. Er ist geeignet, ARTN in Proben von Human zu detektieren.

Kurzübersicht für ARTN Antikörper (ABIN181680)

Target: ARTN (Artemin (ARTN))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARTN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ARTN Antikörper

Spezifität: This antibody detects Artemin.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Highly pure (> 98%) derived recombinant Human Artemin

Anwendungsinformationen

Applikationshinweise: ELISA: Indirect: To detect hArtemin (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanArtemin as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hArtemin. Western blot: To detect hArtemin this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu ARTN

Target: ARTN (Artemin (ARTN))

Andere Bezeichnung: Artemin

Hintergrund: Artemin is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands, a group of ligands within the TGF-beta superfamily of signalling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.Synonyms: ARTN, EVN, Enovin, Neublastin

Gen-ID: 9048

NCBI Accession: NP_001129687

UniProt: Q5T4W7