MIA Antikörper

Produktnummer ABIN181672

Produktdetails anti-MIA Antikörper

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects MIA.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Highly pure (> 98%) recombinant Human MIA

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sectionsof human pancreas infiltrating ductal adenocarcinoma tissue. The recommendedconcentration is 1.0-2.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeledpolymer detecttion system was used with an alcohol-soluble AEC chromogen. Optimalresults for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer enzyme induced antigen retrievalwith proteinase K at roomtemperature.
Optimal conditions may vary. Tissue samples were provided by theCooperative Human Tissue Network which is funded by the National Cancer Institute. Indirect ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human MIA (ABIN181670 or ABIN181669) as a Detection antibody, itallows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA this antibody can be used at a concentration of 0.1-0.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: MIA (MIA Produkte)

Synonyme: CD-RAP antikoerper, Mia1 antikoerper, Cdrap antikoerper, zgc:194157 antikoerper, mia antikoerper, CDRAP antikoerper, Fdp antikoerper, MIA antikoerper, MIAL antikoerper, melanoma inhibitory activity antikoerper, otoraplin antikoerper, cilia and flagella associated protein 100 antikoerper, MIA antikoerper, Mia antikoerper, mia antikoerper, Otor antikoerper, CFAP100 antikoerper

Hintergrund: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

Gen-ID: 8190

NCBI Accession: NP_001189482

UniProt: Q16674