MIA Antikörper (Biotin)

Produktnummer ABIN181669

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MIA in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MIA Antikörper (Biotin) (ABIN181669)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MIA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-MIA Antikörper (Biotin)

Spezifität: This antibody detects MIA.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (> 98%) recombinant Human MIA

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentration ofapprox. 1.0 μg/mL of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. In conjunction with PolyclonalAnti-Human MIA (ABIN181672 or ABIN181671) as a Capture antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: MIA

Hintergrund: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

Gen-ID: 8190

NCBI Accession: NP_001189482

UniProt: Q16674