RELM alpha Antikörper (Biotin)

Produktnummer ABIN181629

Dieser Anti-RELM alpha-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von RELM alpha in WB und EIA. Geeignet für Maus.

Kurzübersicht für RELM alpha Antikörper (Biotin) (ABIN181629)

Target: RELM alpha

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RELM alpha Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-RELM alpha Antikörper (Biotin)

Spezifität: This antibody detects RELM alpha.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine RELMalpha

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect mRELMα by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mRELMα. Sandwich: To detect mRELMα by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine RELMα as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mRELMα. Western Blot: To detect mRELMα by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRELMα is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu RELM alpha

Target: RELM alpha

Hintergrund: Synonyms: Cysteine-rich secreted protein A12-gamma, Cysteine-rich secreted protein FIZZ1, FIZZ1, PMNG1, Parasite-induced macrophage novel gene 1 protein, RELM-alpha, RELMalpha, RETNLA, Resistin-like alpha

Gen-ID: 57262

NCBI Accession: NP_065255

UniProt: Q9EP95