ELISA: Direct: To detect mRELMα by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mRELMα. Sandwich: To detect mRELMα by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine RELMα as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mRELMα. Western Blot: To detect mRELMα by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mRELMα is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.