CCL4 Antikörper (Biotin)

Produktnummer ABIN181625

Der Kaninchen Polyklonal Anti-CCL4-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CCL4 in Proben von Maus zu detektieren.

Kurzübersicht für CCL4 Antikörper (Biotin) (ABIN181625)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL4 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL4 Antikörper (Biotin)

Spezifität: This antibody detects Macrophage Inflammatory Protein-1 beta.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant murine MIP-1beta

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect mMIP-1β by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mMIP-1β. Sandwich: To detect mMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Murine MIP-1β as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant mMIP-1β. Western Blot: To detect mMIP-1β by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMIP-1β is 1.5 - 3.0 ng/lane, under either reducing or

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Andere Bezeichnung: MIP1 beta / CCL4

Hintergrund: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

Gen-ID: 20303

NCBI Accession: NP_038680

UniProt: P14097