IL-19 Antikörper (Biotin)

Produktnummer ABIN181613

Der Kaninchen Polyklonal anti-IL-19 Antikörper wird verwendet zum Nachweis von IL-19 in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für IL-19 Antikörper (Biotin) (ABIN181613)

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-19 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL-19 Antikörper (Biotin)

Spezifität: This antibody detects Interleukin-19.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-19

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hIL-19 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Sandwich: To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human IL-19 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hIL-19. Western Blot: To detect hIL-19 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu IL-19

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Andere Bezeichnung: Interleukin-19 / IL19

Hintergrund: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: IL-19, Melanoma differentiation-associated protein-like protein, NG.1, ZMDA

Gen-ID: 29949

NCBI Accession: NP_037503

UniProt: Q9UHD0