CCL24 Antikörper

Produktnummer ABIN181600

Dieses Anti-CCL24-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von CCL24 in WB, EIA und Func. Geeignet für Human.

Kurzübersicht für CCL24 Antikörper (ABIN181600)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CCL24 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CCL24 Antikörper

Spezifität: This antibody detects Eotaxin-2.

Aufreinigung: Immunoaffinity Chromatography employing an immobilized Human Eotaxin-2 matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human Eotaxin-2.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofEotaxin-2 (100 ng/mL), a concentration of 4.0 - 6.0 μg/mL is required. ELISA: Indirect: To detect Eotaxin-2 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant Eotaxin-2. Sandwich: To detect hEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanEotaxin-2 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Andere Bezeichnung: Eotaxin-2 / CCL24

Hintergrund: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.Synonyms: C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, MPIF-2, MPIF2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24

Gen-ID: 6369

NCBI Accession: NP_002982

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization