Interleukin 17a Antikörper (Biotin)

Produktnummer ABIN181569

Dieses Anti-Interleukin 17a-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Interleukin 17a in WB und EIA. Geeignet für Human.

Kurzübersicht für Interleukin 17a Antikörper (Biotin) (ABIN181569)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Interleukin 17a Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Interleukin 17a Antikörper (Biotin)

Spezifität: This antibody detects Interleukin-17A.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-17A

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hIL-17A by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Sandwich: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanIL-17A as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: Interleukin-17A (IL17A)

Hintergrund: IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17

Gen-ID: 3605

NCBI Accession: NP_002181

UniProt: Q16552