CCL13
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
HRP
Applikationshinweise
ELISA: Indirect: To detect hMCP-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Sandwich: To detect hMCP-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. This In conjunction with BiotinylatedAnti-Human MCP-4 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-4 (100 ng/mL), a concentration of 8 - 12 μg/mL of this antibody is required. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.Synonyms: C-C motif chemokine 13, CCL13, CK-beta-10, MCP4, Monocyte chemotactic protein 4, NCC-1, NCC1, SCYA13, Small-inducible cytokine A13