BMP7 Antikörper

Produktnummer ABIN181552

Dieses Anti-BMP7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von BMP7 in WB, EIA und Func. Geeignet für Human.

Kurzübersicht für BMP7 Antikörper (ABIN181552)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMP7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-BMP7 Antikörper

Spezifität: Detects BMP7.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography using an immobilized Human BMP7 matrix.

Immunogen: CHO cell derived recombinant highly pure (>98%) Human BMP-7.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP7 (100 ng/mL), a concentration of 0.40-0.60 μg/mL of this BMP7 antibody is required. Sandwich ELISA: To detect Human BMP7 by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL is required. The antigen affinity purified BMP7 antibody, inconjunction with Biotinylated antibody anti-Human BMP7 antibody (ABIN181549 orABIN181550) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human BMP-7. Western Blot: To detect Humnan BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Hunan BMP-7 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Andere Bezeichnung: BMP7

Hintergrund: Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1

Gen-ID: 655

NCBI Accession: NP_001710

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance