BMP7 Antikörper (Biotin)

Produktnummer ABIN181549

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BMP7 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für BMP7 Antikörper (Biotin) (ABIN181549)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMP7 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-BMP7 Antikörper (Biotin)

Spezifität: This antibody detects BMP7.

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human BMP-7

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect BMP-7 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant BMP-7. Sandwich: To detect BMP-7 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human BMP-7 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant BMP-7. Western Blot: To detect BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant BMP-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Andere Bezeichnung: BMP7

Hintergrund: Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1

Gen-ID: 655

NCBI Accession: NP_001710

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance