Follistatin Antikörper

Produktnummer ABIN181535

Der Kaninchen Polyklonal anti-Follistatin Antikörper wird verwendet zum Nachweis von Follistatin in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für Follistatin Antikörper (ABIN181535)

Target: Follistatin (FST)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Follistatin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Follistatin Antikörper

Spezifität: This antibody detetcs Follistatin.

Aufreinigung: Affinity purified

Immunogen: Highly pure (> 98 %) recombinant human Follistatin

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect hFollistatin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Sandwich: To detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-HumanFollistatin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hFollistatin. Western Blot: To detect hFollistatin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Details zu Follistatin

Target: Follistatin (FST)

Andere Bezeichnung: Follistatin

Hintergrund: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone. There are three different isoforms, generated by alternative splicing.Synonyms: Activin-binding protein, FS, FST

Gen-ID: 10468

NCBI Accession: NP_006341

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion