Nerve Growth Factor Antikörper

Produktnummer ABIN181153

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nerve Growth Factor in WB, Func und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nerve Growth Factor Antikörper (ABIN181153)

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Produktdetails anti-Nerve Growth Factor Antikörper

Spezifität: This antibody detects NGF-beta.

Produktmerkmale: Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) recombinant Human beta-NGF

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman beta-NGF (10.0 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody isrequired. ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant Human Beta-NGF when used withBiotin anti-Human beta-NGF antibody (cat. PP1057B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can beused at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gen-ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size