PRNP Antikörper

Produktnummer ABIN180947

Der Maus Monoklonal Anti-PRNP-Antikörper wurde für IHC (p) und EIA validiert. Er ist geeignet, PRNP in Proben von Schaf zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für PRNP Antikörper (ABIN180947)

Target: PRNP (Prion Protein (PRNP))

Reaktivität: Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRNP Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 2G11

Produktdetails anti-PRNP Antikörper

Spezifität: This antibody recognises the scrapie-associated protease-resistant isoform (PrPsc) of ovine prion protein (PrP). Clone 2G11 specifically recognises the Re151-Re159 sequence and does not recognise the non-pathogenic PrPec form.

Produktmerkmale: Synonyms: Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP

Aufreinigung: Affinity chromatography on Protein G

Immunogen: Synthetic peptide 146-Re154Re171-182 of ovine PrP

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA: 1/50 - 1/1000, Tested on peptide. Immunohistochemistry on paraffin sections: suitable for use on tissue fixed in 4 % neutralbuffered formalin. Treatment of tissue sections in 98 % formic acid, for 30 minutes, isrecommended prior to pre-treatment with trypsin at 37C for 5 minutes followed by heatmediated retrieval with 10 mM citrate buffer pH 6.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-PRNP Antikörper (ABIN180947)

Reil, Bux: "Geno- and phenotyping of human neutrophil antigens." in: Methods in molecular biology (Clifton, N.J.), Vol. 1310, pp. 193-203, (2016) (PubMed).

Lederle, Hartenstein, Meides, Kunzelmann, Werb, Angel, Mueller: "MMP13 as a stromal mediator in controlling persistent angiogenesis in skin carcinoma." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1175-84, (2010) (PubMed).

Rolhion, Barnich, Bringer, Glasser, Ranc, Hébuterne, Hofman, Darfeuille-Michaud: "Abnormally expressed ER stress response chaperone Gp96 in CD favours adherent-invasive Escherichia coli invasion." in: Gut, Vol. 59, Issue 10, pp. 1355-62, (2010) (PubMed).

Andréoletti, Berthon, Levavasseur, Marc, Lantier, Monks, Elsen, Schelcher: "Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 50, Issue 10, pp. 1357-70, (2002) (PubMed).

Details zu PRNP

Target: PRNP (Prion Protein (PRNP))

Andere Bezeichnung: CD230 / PrP

Hintergrund: The prion protein is encoded by the PrP gene and is constitutively expressed as the normal cellular isoform PrPec by many cell types. Transmissible spongiform encephalopathies (TSE) are neurodegenerative diseases in which the accumulation of the abnormal PrPesc isoform in tissues, of the lymphoid and central nervous system, is thought to be a key event. Studies suggest that PrPesc is the causative agent of transmissible spongiform encephalitis (TSE) but this has not been confirmed.Synonyms: ASCR, Major prion protein, PRIP, PRNP, PrP27-30, PrP33-35C

Gen-ID: 493887

UniProt: P23907

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation