GPR125 Antikörper (AA 1098-1109)

Produktnummer ABIN1804467

Dieses Anti-GPR125-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von GPR125 in WB, ELISA, IHC (p) und IHC. Geeignet für Human, Maus, Rind (Kuh), Fledermaus, Hamster, Pferd, Affe und Kaninchen.

Kurzübersicht für GPR125 Antikörper (AA 1098-1109) (ABIN1804467)

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Reaktivität: Human, Maus, Rind (Kuh), Fledermaus, Hamster, Pferd, Affe, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GPR125 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Produktdetails anti-GPR125 Antikörper

Bindungsspezifität: AA 1098-1109

Marke: IHC-plus™

Spezifität: Human GPR125.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CTNKSASSFKNS from an internal region of human GPR125 (NP_660333.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Bovine, Bat, Horse, Rabbit (100%), Panda, Dog, Pig, Opossum, Turkey, Platypus, Xenopus (92%), Chicken (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GPR125

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Andere Bezeichnung: ADGRA3 / GPR125

Hintergrund: Name/Gene ID: ADGRA3
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRA3, GPR125, TEM5L, G protein-coupled receptor 125, PGR21

Gen-ID: 166647

NCBI Accession: NP_660333

UniProt: Q8IWK6