Moesin Antikörper

Produktnummer ABIN1803989

Der Maus Monoklonal Anti-Moesin-Antikörper wurde für WB, IHC, IF, ELISA, IP und IHC (p) validiert. Er ist geeignet, Moesin in Proben von Human, Ratte und Rind (Kuh) zu detektieren.

Kurzübersicht für Moesin Antikörper (ABIN1803989)

Target: Moesin (MSN)

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Moesin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Moesin Antikörper

Marke: IHC-plus™

Spezifität: Recognizes 78kD moesin. Weakly recognizes 80kD radixin. Detects most of the proteolytic fragments of moesin. Does not react with talin or ezrin.

Aufreinigung: Protein G purified

Immunogen: Moesin from cow uterus.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: Denatured only. 2 μg/mg protein lysate using Protein G. Western Blot: 1-2 μg/mL for 2 hours at RT. Positive control: Raji cells, placenta.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Moesin

Target: Moesin (MSN)

Andere Bezeichnung: MSN / Moesin

Hintergrund: Name/Gene ID: MSN

Synonyms: MSN, Moesin

Gen-ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization