Profilin Antikörper (AA 42-47)

Produktnummer ABIN1742560

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Profilin in WB, ICC und IP. Er zeigt eine Reaktivität gegenüber Birch und wurde in 1 Publikation erwähnt.

Kurzübersicht für Profilin Antikörper (AA 42-47) (ABIN1742560)

Target: Profilin (PFN)

Reaktivität: Birch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Profilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 4A6

Produktdetails anti-Profilin Antikörper

Bindungsspezifität: AA 42-47

Spezifität: Specific for birch profilin.

Aufreinigung: purified IgG

Immunogen: Recombinant birch profilin. Epitope PQFKPQ (aa 42-47)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 10000
IHC: not tested yet

Kommentare:

This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-Profilin Antikörper (ABIN1742560)

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Details zu Profilin

Target: Profilin (PFN)

Andere Bezeichnung: Profilin