Profilin Antikörper

Produktnummer ABIN1742559

Dieses Anti-Profilin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Profilin in WB, ICC und IHC. Geeignet für Human, Rind (Kuh), Meerschweinchen und Kaninchen. Dieses Primary Antibody wurde in 6+ Publikationen zitiert.

Kurzübersicht für Profilin Antikörper (ABIN1742559)

Target: Profilin (PFN)

Reaktivität: Human, Rind (Kuh), Meerschweinchen, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Profilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Klon: 2H11

Produktdetails anti-Profilin Antikörper

Spezifität: Specific for profilin 1.

Aufreinigung: purified IgG

Immunogen: Fusion protein of alpha-actinin from dictyostelium and bovine profilin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 5000 (AP staining)
IP: not tested yet

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-Profilin Antikörper (ABIN1742559)

Lorente, Syriani, Morales: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." in: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).

Giesemann, Schwarz, Nawrotzki, Berhörster, Rothkegel, Schlüter, Schrader, Schindelin, Mendel, Kirsch, Jockusch: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).

Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).

Janke, Schlüter, Jandrig, Theile, Kölble, Arnold, Grinstein, Schwartz, Estevéz-Schwarz, Schlag, Jockusch, Scherneck: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." in: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).

Schlüter, Schleicher, Jockusch: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." in: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).

Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).

Details zu Profilin

Target: Profilin (PFN)

Andere Bezeichnung: Profilin