alpha Tubulin Antikörper (AA 419-435)

Produktnummer ABIN1742547

Dieses Anti-alpha Tubulin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von alpha Tubulin in WB, ELISA, IP und ICC. Geeignet für Human, Maus, Ratte, Saccharomyces cerevisiae, Vertebrate und Invertebrat. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für alpha Tubulin Antikörper (AA 419-435) (ABIN1742547)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae, Vertebrate, Invertebrat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 3A2

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: AA 419-435

Spezifität: Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200

Kommentare:

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-alpha Tubulin Antikörper (ABIN1742547)

Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, (2013) (PubMed).

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Pathways: Microtubule Dynamics