GABRA1 Antikörper (N-Term)

Produktnummer ABIN1742457

Dieser Anti-GABRA1 Antikörper ist ein Meerschweinchen Polyklonal Antikörper zur Detektion von GABRA1 in WB und ICC. Geeignet für Ratte und Maus.

Kurzübersicht für GABRA1 Antikörper (N-Term) (ABIN1742457)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reaktivität: Ratte, Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Güteklasse: KO Validated

Produktdetails anti-GABRA1 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: anti-GABA-A receptor alpha1

Spezifität: Specific for GABA-A receptor alpha1.

Kreuzreaktivität (Details): Reacts with: rat (P62813), mouse (P62812). Other species not tested yet.

Aufreinigung: affinity purified

Immunogen: Synthetic peptide/recombinant protein corresponding to residues near the amino terminus from rat GABA-A receptor alpha1. (UniProt Id: P62813)

Anwendungsinformationen

Applikationshinweise: WB:1 : 1000 up to 1 : 2000 , IHC-P:not tested yet

Kommentare:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Lagerung: -20 °C,-80 °C,4 °C

Informationen zur Lagerung: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Details zu GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Andere Bezeichnung: GABA-A receptor alpha1

Hintergrund: GABA receptor, Gabra1

UniProt: P62813