Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) (AA 4-232) Antikörper

Details zu Produkt Nr. ABIN1742386
Antigen
Epitop
AA 4-232
6
2
1
1
1
1
Reaktivität
Human, Ratte (Rattus), Maus
49
31
26
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
41
7
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
27
15
13
8
6
3
2
2
1
1
Optionen
Immunogen Recombinant human p 31 (aa 4-232).
Spezifität Specific for p 31.
Reinigung Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Antigen
Andere Bezeichnung P 31 (PSMD8 Antibody Abstract)
Hintergrund Synonyms: Use 1
Pathways Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA, SARS-CoV-2 Protein Interaktom
Applikations-hinweise WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 200
IHC: not tested yet
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handhabung Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Lagerung -20 °C
Informationen zur Lagerung Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Bilder des Herstellers
Image no. 1 for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) (AA 4-232) antibody (ABIN1742386) dilution: 1 : 1000, sample: cell lysate from Jurkat cells
Image no. 2 for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8) (AA 4-232) antibody (ABIN1742386) Indirect immunofluorescence on 3T3 cells (dilution 1 : 200; red). The antibody stains...
Produkt verwendet in: Nieradka, Ufer, Thiadens, Grech, Horos, van Coevorden-Hameete, van den Akker, Sofi, Kuhn, von Lindern: "Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment." in: PLoS ONE, Vol. 9, Issue 9, pp. e104631, 2014 (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, 2006 (PubMed).