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VAMP5 Antikörper (AA 1-70)

Der Kaninchen Polyklonal Anti-VAMP5-Antikörper wurde für IHC, WB und ICC validiert. Er ist geeignet, VAMP5 in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.
Produktnummer ABIN1742369

Kurzübersicht für VAMP5 Antikörper (AA 1-70) (ABIN1742369)

Target

Alle VAMP5 Antikörper anzeigen
VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

Reaktivität

  • 19
  • 5
  • 3
Maus

Wirt

  • 17
  • 4
  • 1
Kaninchen

Klonalität

  • 20
  • 3
Polyklonal

Konjugat

  • 13
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser VAMP5 Antikörper ist unkonjugiert

Applikation

  • 10
  • 9
  • 8
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 6
    • 3
    • 2
    • 1
    • 1
    AA 1-70

    Spezifität

    Specific for myobrevin.

    Aufreinigung

    Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

    Immunogen

    Recombinant mouse myobrevin (aa 1-70).
  • Applikationshinweise

    WB: 1 : 1000 up to 1 : 5000 (AP staining)
    IP: not tested yet
    ICC: 1 : 1000
    IHC-P: 1 : 200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

    Buffer

    PBS

    Handhabung

    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
  • Ikezawa, Tajika, Ueno, Murakami, Inoue, Yorifuji: "Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2017) (PubMed).

    Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, (2015) (PubMed).

    Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." in: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, (2013) (PubMed).

    Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, (2009) (PubMed).

  • Target

    VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

    Andere Bezeichnung

    Myobrevin

    Hintergrund

    Synonyms: VAMP 5
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