UNC13A/Munc13-1 Antikörper (AA 3-317)

Produktnummer ABIN1742277

Dieser Anti-UNC13A/Munc13-1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von UNC13A/Munc13-1 in WB, ICC, IP und IHC. Geeignet für Human, Maus, Ratte und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für UNC13A/Munc13-1 Antikörper (AA 3-317) (ABIN1742277)

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UNC13A/Munc13-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Güteklasse: KO Validated

Produktdetails anti-UNC13A/Munc13-1 Antikörper

Bindungsspezifität: AA 3-317

Verwendungszweck: Rabbit anti-Munc13-1 antiserum

Spezifität: Specific for munc 13-1.

Kreuzreaktivität (Details): Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.

Aufreinigung: antiserum

Immunogen: Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)

Anwendungsinformationen

Applikationshinweise: WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 200, IHC-P:not tested yet

Kommentare:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handhabung: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

Referenzen für anti-UNC13A/Munc13-1 Antikörper (ABIN1742277)

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

Details zu UNC13A/Munc13-1

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Andere Bezeichnung: Munc13-1

Hintergrund:

Synonyms: Munc13-1, Munc13, Protein unc-13 homolog A, Unc13a, Unc13h1, Brain, Hippocampus, Cerebellum, Olfactory bulb, Cortex, Striatum

Antigen Information: K.O. Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis

UniProt: Q62768

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis