UNC13A/Munc13-1 Antikörper (AA 3-317)

Produktnummer ABIN1742277

Dieses Anti-UNC13A/Munc13-1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von UNC13A/Munc13-1 in WB, IP, ICC und IHC. Geeignet für Human, Maus, Ratte und Zebrafisch (Danio rerio). Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für UNC13A/Munc13-1 Antikörper (AA 3-317) (ABIN1742277)

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UNC13A/Munc13-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Produktdetails anti-UNC13A/Munc13-1 Antikörper

Bindungsspezifität: AA 3-317

Spezifität: Specific for munc 13-1.

Aufreinigung: antiserum

Immunogen: Recombinant rat munc 13-1 (aa 3-317).

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200
ELISA: not tested yet

Kommentare:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

Buffer: PBS

Handhabung: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-UNC13A/Munc13-1 Antikörper (ABIN1742277)

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

Details zu UNC13A/Munc13-1

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Andere Bezeichnung: Munc 13-1

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis