SV2B Antikörper (AA 2-17)

Produktnummer ABIN1742257

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SV2B in WB, IHC, ICC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für SV2B Antikörper (AA 2-17) (ABIN1742257)

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SV2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: 246E8

Produktdetails anti-SV2B Antikörper

Bindungsspezifität: AA 2-17

Spezifität: Specific for SV2 B.

Aufreinigung: purified IgG

Immunogen: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200
IHC-P: 1 : 500

Kommentare:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide (= 0.02 µg)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-SV2B Antikörper (ABIN1742257)

Aroeira, Vaz, Sebastião, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).

Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).

Details zu SV2B

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Andere Bezeichnung: SV2 B