Clathrin Antikörper (AA 156-173)

Produktnummer ABIN1742245

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Clathrin in IHC, WB und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Zebrafisch (Danio rerio) und wurde in 11+ Publikationen erwähnt.

Kurzübersicht für Clathrin Antikörper (AA 156-173) (ABIN1742245)

Target: Clathrin

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Clathrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Klon: 57-4

Produktdetails anti-Clathrin Antikörper

Bindungsspezifität: AA 156-173

Spezifität: Specific for both neuronal light chains

Kreuzreaktivität (Details): does not cross-react with the non-neuronal variants.

Aufreinigung: purified IgG

Immunogen: Synthetic peptide IADKAFYQQPDADTIGYV (aa 156-173) corresponding to the spliced insert of the neuronal light chain variants.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 : 5000 up to 1 : 10000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC-P: 1 : 2000

Kommentare:

Suitable for immunogold electron microscopy.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen für anti-Clathrin Antikörper (ABIN1742245)

Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Hellwig, Hack, Kowalski, Brunne, Jarowyj, Unger, Bock, Junghans, Frotscher: "Role for Reelin in neurotransmitter release." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 7, pp. 2352-60, (2011) (PubMed).

Ferguson, Brasnjo, Hayashi, Wölfel, Collesi, Giovedi, Raimondi, Gong, Ariel, Paradise, Otoole, Flavell, Cremona, Miesenböck, Ryan, De Camilli: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).

Krauss, Kukhtina, Pechstein, Haucke: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, (2006) (PubMed).

Johnson, Brown, Pulliam, Anderson, Johnson: "Synaptic pathology, altered gene expression, and degeneration in photoreceptors impacted by drusen." in: Investigative ophthalmology & visual science, Vol. 46, Issue 12, pp. 4788-95, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Blumstein, Faundez, Nakatsu, Saito, Ohno, Kelly: "The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 20, pp. 8034-42, (2001) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Details zu Clathrin

Target: Clathrin